Coding

Part:BBa_K1903008:Design

Designed by: Sofía Vieto Fonseca   Group: iGEM16_TEC-Costa_Rica   (2016-10-14)


Split TEV-IntC DnaE Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 172
    Illegal SapI.rc site found at 482


Design Notes

This part is a device created with the split Intein C terminal from Npu DnaE and the split TEV C terminal BBa_K11903007, fused to the Anderson promoter BBa_J23101 assembled with the RBS Elowitz BBa_B0034.

We assembled this device and verified it in an agarose gel electrophoresis:



We were able to express the intein C terminal from Npu DnaE and the split TEV C terminal in the following SDS-PAGE:


Source

References